Reproducibility of random amplified polymorphic DNA (RAPD) analysis among laboratories.
نویسندگان
چکیده
منابع مشابه
Random Amplified Polymorphic DNA (RAPD) Markers
Due to advances in molecular biology techniques, large numbers of highly informative DNA markers have been developed for the identification of genetic polymorphism. In the last decade, the random amplified polymorphic DNA (RAPD) technique based on the polymerase chain reaction (PCR) has been one of the most commonly used molecular techniques to develop DNA markers. RAPD markers are amplificatio...
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Ten field isolates of Mycoplasma gallisepticum (MG) from different geographical areas of Iran were analyzed by random amplification of polymorphic DNA (RAPD). RAPD analysis produced reproducible banding patterns on the basis of which various distinct amplification patterns could be detected. MG isolates compared with reference strains (S6 and Mg SS) and vaccine strain (ts-11) and demonstrated...
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Forensic entomology is the study of insects found around the crime scene and are helpful for the estimation of time elapsed since death of a person i.e., post mortem interval (PMI). There are number techniques available for the identification of insect species and Random Amplified Polymorphic DNA (RAPD) is one of those. RAPD is a useful technique designed for the detection of sequence polymorph...
متن کاملRandom amplified polymorphic DNA (RAPD) markers and its applications
In the last decade, the random amplified polymorphic DNA (RAPD) technique based on the polymerase chain reaction (PCR) has been one of the most commonly used molecular techniques to develop DNA markers. RAPD is a modification of the PCR in which a single, short and arbitrary oligonucleotide primer, able to anneal and prime at multiple locations throughout the genome, can produce a spectrum of a...
متن کاملOptimizing reproducibility evaluation for random amplified polymorphic DNA markers.
The random amplified polymorphic DNA (RAPD) technique is often criticized because it usually shows low levels of repeatability; thus it can generate spurious bands. These problems can be partially overcome by rigid laboratory protocols and by performing repeatability tests. However, because it is expensive and time-consuming to obtain genetic data twice for all individuals, a few randomly chose...
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ژورنال
عنوان ژورنال: Genome Research
سال: 1993
ISSN: 1088-9051
DOI: 10.1101/gr.2.4.341